Albany Medical College
Department of Molecular and Cellular Physiology (MCP)
My research has been focused on defining molecular mechanisms controlling vascular remodeling, including vascular smooth muscle (VSMC) phenotypic modulation which underlies diverse vascular pathologies. We implement in vitro primary vascular smooth muscle cell culture, human blood vessel ex vivo culture system, and genetic mouse models to comprehensively dissect functional roles of novel genes such as lncRNAs in various vascular diseases including restenosis, arteriovenous fistula (AVF) failure, and aneurysm.
Vascular disease models: femoral artery wire injury, carotid artery ligation, aneurysm, arteriovenous fistula (AVF).
Transcriptional regulation: in vitro reporter assay, Chromatin Immunoprecipitation assays (ChIPs), Chromatin Immunoprecipitation Sequencing (ChIP-Seq), CRISPR-Cas genome editing critical cis-regulatory elements in mice.
lncRNA biology: RNA Immunoprecipitation (RIP), in vitro RNA pulldown, immunofluorescence-based double staining of RNA and protein.
Interested in collaborating on
RNA structure, human sample studies on aneurysm and pulmonary artery hypertension.
Selected publications on lncRNA
MYOSLID is a novel serum response factor-dependent long non-coding RNA that amplifies the vascular smooth muscle differentiation program
Zhao J, Zhang W, Lin M, Wu W, Jiang P, Tou E, Xue M, Richards A, Jourd'heuil D, Asif A, Zheng D, Singer HA, Miano JM, Long X. Arterioscler Thromb Vasc Biol. 2016, 36(10):2088-99.
The short and long of noncoding sequences in the control of vascular cell phenotypes
Miano JM and Long X. Cell Mol Life Sci. 2015, 72(18):3457-88.
Identification and initial functional characterization of a human vascular cell-enriched long noncoding RNA
Bell RD, Long X, Lin M, Bergmann JH, Nanda V, Cowan SL, Zhou Q, Han Y, Spector DL, Zheng D, Miano JM. Arterioscler Thromb Vasc Biol. 2014, 34(6):1249-59.