The seminar entitled "Potent knock down of lncRNAs in vitro and in vivo with antisense LNA™ GapmeRs" will be held in Luxembourg on November 25, 2016.
One of the most basic yet powerful approaches to study lncRNAs is loss of function analysis, but since many lncRNAs are nuclear retained or have long residence time in the nucleus, these are hard to target by RNAi based methods.
Exiqon has developed single stranded LNA™-enhanced antisense oligonucleotides (ASOs, also known as LNA™ GapmeRs) that catalyze RNaseH dependent degradation of both mRNAs and lncRNA. Since RNaseH is almost exclusively present in the nucleus, all RNAs are potentially sensitive to LNA™ GapmeR knock down.
To address potential off targets located in either exons or introns, the LNA™ GapmeR design algorithm searches both spliced and unspliced transcriptomes in the Ensembl database, to provide maximal target specificity.
Both mRNA and lncRNA targets residing in either cytoplasmic or nuclear compartments are equally efficiently silenced irrespective of the type of RNA target and its subcellular localization.
For more information about the seminar, visit our events page.