University of Lausanne Medical School

Experimental Cardiology Unit

 

Thierry Pedrazzini
Samir Ounzain

Enhancer-associated lncRNAs are key determinants of lineage specification during development. They also play crucial roles in controlling cell identity and behavior during cellular responses to diseased states. We are currently evaluating remodeling of the enhancer landscape and modulation of the lncRNA transcriptome associated with it during the adaptation of the heart to hemodynamic stress, such as that occurring following myocardial infarction. In this context, we investigate various approaches to reprogram cardiac cells in an attempt to promote regeneration in
the damaged myocardium.

Expertise

  • Assessment of the enhancer-associated lncRNA transcriptome in cardiac cells
  • Transcriptomic approach
  • Bioinformatic reconstruction of the lncRNA transcriptome
  • Combined analysis including integration of epigenetic data
  • Mouse models of cardiac diseases
  • Functional assessment of the cardiovascular system
  • Isolation of mouse cardiac myocytes and non-myocyte cells

Interested in collaborating on

Expertise in assessing molecular mechanisms associated to lncRNA functions

Selected publications  on ncRNA and cardiovascular disease

A transcribed enhancer dictates mesendoderm specification in pluripotency
Alexanian M, Maric D, Jenkinson SP, Mina M, Friedman CE, Ting CC, Micheletti R, Plaisance I, Nemir M, Maison D, Kernen J, Pezzuto I, Villeneuve D, Burdet F, Ibberson M, Leib SL, Palpant NJ, Hernandez N, Ounzain SPedrazzini TNat Commun. 2017, 8(1): 1806.

The long noncoding RNA Wisper controls cardiac fibrosis and remodeling
Micheletti R, Plaisance I, Abraham BJ, Sarre A, Ting CC, Alexanian M, Maric D, Maison D, Nemir M, Young RA, Schroen B, González A, Ounzain S, Pedrazzini TSci Transl Med. 2017, 9(395). pii: eaai9118.

Cardiomyocyte Lineage Specification in Adult Human Cardiac Precursor Cells Via Modulation of Enhancer-Associated Long Noncoding RNA Expression
Plaisance I, Perruchoud S, Fernandez-Tenorio M, Gonzales C, Ounzain S, Ruchat P, Nemir M, Niggli E, Pedrazzini T. JACC: Basic to Translational Science. 2016, 1(6):472-493.

CARMEN, a human super enhancer-associated long noncoding RNA controlling cardiac specification, differentiation and homeostasis
Ounzain S, Micheletti R, Arnan C, Plaisance I, Cecchi D, Schroen B, Reverter F, Alexanian M, Gonzales C, Ng SY, Bussotti G, Pezzuto I, Notredame C, Heymans S, Guigó R, Johnson R, Pedrazzini TJ Mol Cell Cardiol. 2015, 89(Pt A):98-112.

Genome-wide profiling of the cardiac transcriptome after myocardial infarction identifies novel heart-specific long non-coding RNAs
Ounzain S, Micheletti R, Beckmann T, Schroen B, Alexanian M, Pezzuto I, Crippa S, Nemir M, Sarre A, Johnson R, Dauvillier J, Burdet F, Ibberson M, Guigó R, Xenarios I, Heymans SPedrazzini TEur Heart J. 2014, 36(6):353-68a.

Functional importance of cardiac enhancer-associated noncoding RNAs in heart development and disease
Ounzain S, Pezzuto I, Micheletti R, Burdet F, Sheta R, Nemir M, Gonzales C, Sarre A, Alexanian M, Blow MJ, May D, Johnson R, Dauvillier J, Pennacchio LA, Pedrazzini TJ Mol Cell Cardiol. 2014, 76:55-70.

Website
thierry.pedrazzini@chuv.ch
samir.ounzain@chuv.ch

Rue du Bugnon 27
CH-1011 Lausanne
Switzerland