University of Rochester
School of Medicine and Dentistry
For the last five years, we have performed RNA-seq in human vascular cells and in silico screening to define new lncRNAs. We published the first vascular-restricted human lncRNA called SENCR and have humanized mice with a BAC harboring SENCR and its neigboring protein-coding gene called FLI1. We utilize an array of biochemical (eg, ChIRP, RIP-seq) and imaging (single cell RNA-FISH) tools to gain a deep understanding of lncRNA function and localization. Finally, for more than four years now, we have exploited the revolutionary CRISPR-Cas9 genome editing platform to elucidate non-coding functions in the mouse genome.
CRISPR-Cas9 genome editing in the mouse (KO, KI, conditional, etc)
ChIRP, RIP-seq, PAR-CLIP
- Defining enhancers, TFBS, and lncRNAs
Interested in collaborating on
- Non-coding functions of coding mRNAs
- Samples of human tissues from patients with various disease
- Circulating lncRNAs in exosomes
- Combined RNA-FISH and immunofluorescence microscopy
Selected publications on lncRNA
Challenges and opportunities of linking long noncoding RNAs to cardiovascular, lung, and blood diseases
Freedman JE and Miano JM. Arterioscler Thromb Vasc Biol. 2017, 37:21-25.
MYOSLID is a novel serum response factor-dependent long non-coding RNA that amplifies the vascular smooth muscle differentiation program
Zhao J, Zhang W, Lin M, Wu W, Jiang P, Tou E, Xue M, Richards A, Jourd'heuil D, Asif A, Zheng D, Singer HA, Miano JM, Long X. Arterioscler Thromb Vasc Biol. 2016, 36(10):2088-99.
Smooth muscle enriched long non-coding RNA (SMILER) regulates cell proliferation
Ballantyne, MD, Dakin R, Pinel K, Vesey AT, Diver L, Mackenze R, Garcia R, Welsh P, Sattar N, Hamilton G, Joshi N, Dweck MR, Miano JM, Newby DE, McDonald RA, Baker AH. Circulation. 2016, 133(21):2050-65.
The short and long of noncoding sequences in the control of vascular cell phenotypes
Miano JM and Long X. Cell Mol Life Sci. 2015, 72(18):3457-88.
Identification and initial functional characterization of a human vascular cell-enriched long noncoding RNA
Bell RD, Long X, Lin M, Bergmann JH, Nanda V, Cowan SL, Zhou Q, Han Y, Spector DL, Zheng D, Miano JM. Arterioscler Thromb Vasc Biol. 2014, 34(6):1249-59.