University of Valencia

Department of Physiology, Laboratorio de Investigación en Células Endoteliales

INCLIVA Biomedical Research Institute


Carlos Hermenegildo

Laboratorio de Investigación en Células Endoteliales (L.In.CE) research group is a multidisciplinary team of expert researchers in Vascular and Endocrine Physiology, Molecular Biology, Biochemistry, Cell Biology and Reproductive Biology.

Main lines of research: 

  • Gender differences in cardiovascular area

  • Vascular effects of sex hormones

  • Identification of new hormone-regulated signalling pathways in endothelium

  • Interaction of sex hormones with pro-atherogenic factors

  • miRNA profile in vascular cells under estrogenic exposure


We perform studies on gene and protein expression, vasoactive substance production measurement and functional approaches on cardiovascular pathophysiology at three levels:

  • Cultured endothelial cells

  • Experimental animal models (mainly senescence accelerated mouse and experimental menopause)

  • Human samples


  • Molecular biology techniques: PCR, RT-PCR, microarrays…

  • Biochemical techniques: western blot, immunofluorescence, ELISA, HPLC…

  • Cell biology techniques: primary cultures of human endothelial and smooth muscle cells, flow cytometry…

  • Vascular physiology techniques: vascular reactivity

Interested in collaborating on

  • To discover and to analyse the disease mechanisms at the vascular level.

  • To study the endothelial and vascular repercussion of the diseases and treatments by means of studying the miRNA, mRNA and protein expression profiles and the endothelial functionality.

  • To discover the implication of sex and gender differences on the vascular consequences of diseases and therapeutic interventions.

  • To identify biomarkers of cardiovascular diseases.

  • To share our collaboration with clinicians and our access to large cohorts of controls and patients with cardiovascular diseases

Selected publications on ncRNA and cardiovascular disease

miRNA as a New Regulatory Mechanism of Estrogen Vascular Action
Pérez-Cremades D, Mompeón A, Vidal-Gómez X, Hermenegildo C, Novella S. Int J Mol Sci. 2018, 19(2). pii: E473.

MicroRNA as Crucial Regulators of Gene Expression in Estradiol-Treated Human Endothelial Cells
Vidal-Gómez X, Pérez-Cremades D, Mompeón A, Dantas AP, Novella S, Hermenegildo CCell Physiol Biochem. 2018, 45(5):1878-1892.


Av. Blasco Ibanez 15
E46010 Valencia