University of Valencia

Department of Physiology, Laboratorio de Investigación en Células Endoteliales

INCLIVA Biomedical Research Institute


Carlos Hermenegildo

Laboratorio de Investigación en Células Endoteliales (L.In.CE) research group is a multidisciplinary team of expert researchers in Vascular and Endocrine Physiology, Molecular Biology, Biochemistry, Cell Biology and Reproductive Biology.

Main lines of research: 

  • Gender differences in cardiovascular area
  • Vascular effects of sex hormones
  • Identification of new hormone-regulated signalling pathways in endothelium
  • Interaction of sex hormones with pro-atherogenic factors
  • miRNA profile in vascular cells under estrogenic exposure


We perform studies on gene and protein expression, vasoactive substance production measurement and functional approaches on cardiovascular pathophysiology at three levels:

  • Cultured endothelial cells
  • Experimental animal models (mainly senescence accelerated mouse and experimental menopause)
  • Human samples


  • Molecular biology techniques: PCR, RT-PCR, microarrays…
  • Biochemical techniques: western blot, immunofluorescence, ELISA, HPLC…
  • Cell biology techniques: primary cultures of human endothelial and smooth muscle cells, flow cytometry…
  • Vascular physiology techniques: vascular reactivity

Interested in collaborating on

  • To discover and to analyse the disease mechanisms at the vascular level.
  • To study the endothelial and vascular repercussion of the diseases and treatments by means of studying the miRNA, mRNA and protein expression profiles and the endothelial functionality.
  • To discover the implication of sex and gender differences on the vascular consequences of diseases and therapeutic interventions.
  • To identify biomarkers of cardiovascular diseases.
  • To share our collaboration with clinicians and our access to large cohorts of controls and patients with cardiovascular diseases


Av. Blasco Ibanez 15
E46010 Valencia